Flotillin-1 Antibody (23GB840)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51977
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB840
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Flotillin-1 (Uniprot: O75955)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Flotillin-1 Antibody (23GB840)
Immunocytochemistry/ Immunofluorescence: Flotillin-1 Antibody (23GB840)[NBP3-51977] -
Immunocytochemical staining of HepG2 cells with Flotillin-1 antibody (1:1,000). Nuclei were stained blue with DAPI; Flotillin-1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Immunocytochemistry/ Immunofluorescence: Flotillin-1 Antibody (23GB840)[NBP3-51977] -
Flotillin-1 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: Flotillin 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Flotillin 1 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
Flow Cytometry: Flotillin-1 Antibody (23GB840)[NBP3-51977] -
Validation of Flotillin-1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Flotillin-1 antibody (1:2,000) and analyzed using CytoFLEX.Applications for Flotillin-1 Antibody (23GB840)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Flotillin-1
Alternate Names
flotillin 1, flotillin-1, integral membrane component of caveolae
Gene Symbol
FLOT1
Additional Flotillin-1 Products
Product Documents for Flotillin-1 Antibody (23GB840)
Product Specific Notices for Flotillin-1 Antibody (23GB840)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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