Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all FKBP15 Antibodies »

Product Specifications

Immunogen

A synthetic peptide mapping to a region between residues 1175 and 1234 (C-terminus) of human FK506-binding protein 135 using the numbering given in TrEMBL entry Q9Y4D0 (GeneID 23307).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FKBP15 Antibody - BSA Free

Immunoprecipitation: FKBP15 Antibody [NB100-424]

Immunoprecipitation: FKBP15 Antibody [NB100-424]

Immunoprecipitation: FKBP15 Antibody [NB100-424] - Detection of human FKBP-135 in immunoprecipitation, using NB100-424. Samples: HeLa nuclear extracts. Exposure time of 10 minutes.
Immunoprecipitation: FKBP15 Antibody [NB100-424]

Immunoprecipitation: FKBP15 Antibody [NB100-424]

Immunoprecipitation: FKBP15 Antibody [NB100-424] - Detection of human FKBP-135 by western blot and immunoprecipitation. Samples: Nuclear extract (NE; 1 mg for IP; 25 ug for WB) from HeLa cells. Antibodies: Affinity purified rabbit anti-FKBP-135 antibodies BL1008 (NB100-422), BL1009 (NB100-423) and BL1010 (NB100-424) used at 2 ug/mg extract for IP. FKBP-135 in NE and immunoprecipitated FKBP-135 were detected using BL1008 (NB100-422) at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 10 minutes.

Applications for FKBP15 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg of lysate
Application Notes
Immunoprecipitation. Western Blot is not recommended with this antibody. NB 100-422 and NB 100-423 are recommended for Western Blot. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FKBP15

Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins.

Alternate Names

FK506 binding protein 15, 133kDa, FK506-binding protein 133kDa, FK506-binding protein 15, FKBP133, FKBP-133, FKBP-15,133 kDa FKBP, PPP1R76,133 kDa FK506-binding protein, regulatory subunit 76, WAFL

Entrez Gene IDs

23307 (Human)

Gene Symbol

FKBP15

UniProt

Q9Y4D0 (Human)

Additional FKBP15 Products

Product Documents for FKBP15 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FKBP15 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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