FKBP15 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-422
![Western Blot: FKBP15 Antibody [NB100-422]](https://resources.bio-techne.com/images/products/FKBP15-Antibody-Western-Blot-NB100-422-img0005.jpg "Western Blot: FKBP15 Antibody [NB100-422]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide mapping to a region between residues 50 and 100 of human FK506-binding protein 135 using the numbering given in TrEMBL entry Q9Y4D0 (GeneID 23307).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit FKBP15 Antibody - BSA Free (NB100-422) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for FKBP15 Antibody - BSA Free
Western Blot: FKBP15 Antibody [NB100-422]
Western Blot: FKBP15 Antibody [NB100-422] - Detection of Human and Mouse FKPB-135 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FKPB-135 antibody NB100-422 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: FKBP15 Antibody [NB100-422]](https://resources.bio-techne.com/images/products/FKBP15-Antibody-Immunoprecipitation-NB100-422-img0006.jpg "Immunoprecipitation: FKBP15 Antibody [NB100-422]")
Immunoprecipitation: FKBP15 Antibody [NB100-422]
Immunoprecipitation: FKBP15 Antibody [NB100-422] - Detection of human FKBP15 (FKPB-135) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FKBP15 antibody NB100-422 (lot 2) used for IP at 6 ug per reaction. FKBP15 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-FKBP15 antibody NB100-423. For blotting immunoprecipitated FKBP15, NB100-422 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for FKBP15 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2- 10 ug/mg of lysate
Western Blot
1:2000-1:10000
Application Notes
Western Blot and immunoprecipitation. *The investigator should determine the optimal working dilution for a specific application.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: FKBP15
Alternate Names
FK506 binding protein 15, 133kDa, FK506-binding protein 133kDa, FK506-binding protein 15, FKBP133, FKBP-133, FKBP-15,133 kDa FKBP, PPP1R76,133 kDa FK506-binding protein, regulatory subunit 76, WAFL
Entrez Gene IDs
23307 (Human)
Gene Symbol
FKBP15
UniProt
Additional FKBP15 Products
Product Documents for FKBP15 Antibody - BSA Free
Product Specific Notices for FKBP15 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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