FIV Matrix protein p15 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11648
Key Product Details
Species Reactivity
Virus
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of FIV Matrix Protein p15 (Uniprot: P16087).
Reactivity Notes
Feline immunodeficiency virus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity purified antibody is directed against FIV Matrix Protein p15. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for FIV Matrix protein p15 Antibody - BSA Free
![Western Blot: FIV Matrix protein p15 Antibody [NBP3-11648]](https://resources.bio-techne.com/images/products/FIV-Matrix-protein-p15-Antibody-Western-Blot-NBP3-11648-img0001.jpg "Western Blot: FIV Matrix protein p15 Antibody [NBP3-11648]")
Western Blot: FIV Matrix protein p15 Antibody [NBP3-11648]
Western Blot: FIV Matrix protein p15 Antibody [NBP3-11648] - Western blot using affinity purified anti-FIV Matrix Protein p15 to detect p15 in the culture supernatant of FIV-infected feline CrFK cells (lane 2, arrowhead). Lane 1 is an uninfected control. Virions were enriched by ultracentrifugation, lysed, resolved by electrophoresis, and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:10,000 dilution.
FIV Matrix protein p15 Antibody
Western blot using affinity purified anti-FIV Matrix Protein p15 to detect p15 in the culture supernatant of FIV-infected feline CrFK cells (lane 2, arrowhead). Lane 1 is an uninfected control. Virions were enriched by ultracentrifugation, lysed, resolved by electrophoresis, and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:10,000 dilution. Personal Communication, B. Luttge, CCR-NCI, Frederick, MD.Applications for FIV Matrix protein p15 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:5000 - 1:20000
Western Blot
1:1000 - 1:10000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting and suitable for immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to FIV Matrix Protein p15 by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: FIV Matrix protein p15
Additional FIV Matrix protein p15 Products
Product Documents for FIV Matrix protein p15 Antibody - BSA Free
Product Specific Notices for FIV Matrix protein p15 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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