Fibulin 1 Antibody (CL0337) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43893
![Fibulin 1 Antibody (CL0337) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP3-43893]](https://resources.bio-techne.com/images/products/nbp3-43893_mouse-fibulin-1-mab-cl0337-azide-and-bsa-free-28220251235121.jpg "Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP3-43893]")
![Fibulin 1 Antibody (CL0337) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP3-43893]](https://resources.bio-techne.com/images/products/nbp3-43893_mouse-fibulin-1-mab-cl0337-azide-and-bsa-free-2822025128373.jpg "Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP3-43893]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (92%), Rat (93%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0337
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P23142, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse Fibulin 1 Antibody (CL0337) - Azide and BSA Free (NBP2-52918) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Fibulin 1 Antibody (CL0337) - Azide and BSA Free
![Fibulin 1 Antibody (CL0337) - Azide and BSA Free Western Blot: Fibulin 1 Antibody [NBP3-43893]](https://resources.bio-techne.com/images/products/nbp3-43893_mouse-fibulin-1-mab-cl0337-azide-and-bsa-free-282202512163621.jpg "Western Blot: Fibulin 1 Antibody [NBP3-43893]")
Western Blot: Fibulin 1 Antibody [NBP3-43893]
Lane 1: Marker [kDa]Lane 2: Human plasma
![Fibulin 1 Antibody (CL0337) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP3-43893]](https://resources.bio-techne.com/images/products/nbp3-43893_mouse-fibulin-1-mab-cl0337-azide-and-bsa-free-282202512145374.jpg "Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP3-43893]")
Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP3-43893]
Staining of human breast cancer shows moderate positivity in the extracellular matrix of tumor stroma.![Fibulin 1 Antibody (CL0337) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP3-43893]](https://resources.bio-techne.com/images/products/nbp3-43893_mouse-fibulin-1-mab-cl0337-azide-and-bsa-free-28220251391952.jpg "Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP3-43893]")
![Fibulin 1 Antibody (CL0337) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Fibulin 1 Antibody [NBP3-43893]](https://resources.bio-techne.com/images/products/nbp3-43893_mouse-fibulin-1-mab-cl0337-azide-and-bsa-free-28220251335849.jpg "Immunocytochemistry/ Immunofluorescence: Fibulin 1 Antibody [NBP3-43893]")
![Fibulin 1 Antibody (CL0337) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Fibulin 1 Antibody [NBP3-43893]](https://resources.bio-techne.com/images/products/nbp3-43893_mouse-fibulin-1-mab-cl0337-azide-and-bsa-free-282202513245456.jpg "Immunocytochemistry/ Immunofluorescence: Fibulin 1 Antibody [NBP3-43893]")
![Fibulin 1 Antibody (CL0337) - Azide and BSA Free Western Blot: Fibulin 1 Antibody [NBP3-43893]](https://resources.bio-techne.com/images/products/nbp3-43893_mouse-fibulin-1-mab-cl0337-azide-and-bsa-free-282202513314678.jpg "Western Blot: Fibulin 1 Antibody [NBP3-43893]")
![Fibulin 1 Antibody (CL0337) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP3-43893]](https://resources.bio-techne.com/images/products/nbp3-43893_mouse-fibulin-1-mab-cl0337-azide-and-bsa-free-282202513254796.jpg "Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP3-43893]")
Applications for Fibulin 1 Antibody (CL0337) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fibulin 1
Fibulin 1 is a matricellular glycoprotein component of the elastic fiber core in connective tissues. It self-aggregates and additionally binds to several extracellular matrix and cellular proteins including Aggrecan, Versican, Fibronectin, ECM1, NOV/CCN3, and pro-HB-EGF. Fibulin 1c enhances ADAMTS-1 mediated cleavage of Aggrecan and Versican and can itself be cleaved by neutrophil elastase or MMP-13. It functions as a bridge between Fibrinogen and Fibrin, enabling Fibrin attachment to the platelet cell surface via Integrin alpha 2 beta 3. Fibulin 1 inhibits cardiomyocyte proliferation and Fibronectin-mediated cell adhesion and can promote bone formation.
Alternate Names
FBLN1, FIBL1
Gene Symbol
FBLN1
Additional Fibulin 1 Products
Product Documents for Fibulin 1 Antibody (CL0337) - Azide and BSA Free
Product Specific Notices for Fibulin 1 Antibody (CL0337) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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