Fibronectin Antibody (23GB1270)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52014
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB1270
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Fibronectin (Uniprot: P02751)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Fibronectin Antibody (23GB1270)
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody (23GB1270)[NBP3-52014] -
Immunocytochemical staining of C2C12 cells with Fibronectin antibody (1:1,000). Nuclei were stained blue with DAPI; Fibronectin was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Flow Cytometry: Fibronectin Antibody (23GB1270)[NBP3-52014] -
Validation of Fibronectin knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Fibronectin antibody (1:2,000) and analyzed using CytoFLEX.
Flow Cytometry: Fibronectin Antibody (23GB1270)[NBP3-52014] -
Flow cytometric analysis of Fibronectinexpression in C2C12 cells using Fibronectin antibody (1:2,000). Green, isotype control; red, Fibronectin 1.Applications for Fibronectin Antibody (23GB1270)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Fibronectin
Alternate Names
CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF
Gene Symbol
FN1
Additional Fibronectin Products
Product Documents for Fibronectin Antibody (23GB1270)
Product Specific Notices for Fibronectin Antibody (23GB1270)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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