Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43555
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 59D8
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated by using a synthetic heptapeptide from the amino terminus of the beta chain in human fibrin as an immunogen. (Uniprot# P02675)
Specificity
This antibody selectively binds to the amino-terminus of the fibrin beta-chain that becomes exposed after cleavage of fibrinopeptide B by thrombin.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Scientific Data Images for Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free
Immunocytochemistry /Immunofluorescence: Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free [NBP3-43555] -
Immunocytochemistry /Immunofluorescence: Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free [NBP3-43555] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix coverslips stained with NBP3-43555 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43555, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.
Western Blot: Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free [NBP3-43555] -
Western Blot: Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free [NBP3-43555] - Human liver (A), placenta (B), and serum (C) tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with NBP3-43555 at 0.01ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.Applications for Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fibrin beta-chain
Alternate Names
Beta-Fibrinogen, epididymis secretory sperm binding protein Li 78p, fibrinogen beta chain, fibrinogen, B beta polypeptide, HEL-S-78p
Gene Symbol
FGB
Additional Fibrin beta-chain Products
Product Documents for Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free
Product Specific Notices for Fibrin beta-chain Antibody (59D8) - Chimeric - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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