Fibrin beta-chain Antibody (59D8) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43554
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 59D8
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated by using a synthetic heptapeptide from the amino terminus of the beta chain in human fibrin as an immunogen. (Uniprot# P02675)
Specificity
This antibody selectively binds to the amino-terminus of the fibrin beta-chain that becomes exposed after cleavage of fibrinopeptide B by thrombin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse Fibrin beta-chain Antibody (59D8) - BSA Free (NBP3-43498) is a recombinant monoclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Fibrin beta-chain Antibody (59D8) - BSA Free
Western Blot: Fibrin beta-chain Antibody (59D8) - BSA Free [NBP3-43554] -
Western Blot: Fibrin beta-chain Antibody (59D8) - BSA Free [NBP3-43554] - Human liver (A), placenta (B), and serum (C) tissue lysates (35µg protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with NBP3-43554 at 0.01µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Fibrin beta-chain Antibody (59D8) - BSA Free [NBP3-43554] -
Immunocytochemistry/ Immunofluorescence: Fibrin beta-chain Antibody (59D8) - BSA Free [NBP3-43554] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on coverslips stained with NBP3-43554 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43554, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.Applications for Fibrin beta-chain Antibody (59D8) - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fibrin beta-chain
Alternate Names
Beta-Fibrinogen, epididymis secretory sperm binding protein Li 78p, fibrinogen beta chain, fibrinogen, B beta polypeptide, HEL-S-78p
Gene Symbol
FGB
Additional Fibrin beta-chain Products
Product Documents for Fibrin beta-chain Antibody (59D8) - BSA Free
Product Specific Notices for Fibrin beta-chain Antibody (59D8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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