Fibrillarin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46881

Novus Biologicals, part of Bio-Techne
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NBP2-46881-0.025ml
NBP2-46881
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Equine

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Fibrillarin Antibodies »

Product Specifications

Immunogen

Full length human Fibrillarin expressed in and purified from E.Coli. [UniProt# P22087]

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

34.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This antibody is supplied as a concentrated total IgY preparation derived from egg yolk. Production involves an initial organic extraction to remove lipids and lipoproteins, followed by salt fractionation of the aqueous phase to precipitate and enrich the IgY fraction. The material is then extensively dialyzed against PBS, and preservative is added during final formulation.

Please note that the exact concentration of target-specific IgY cannot be determined, as the final preparation contains both antigen-specific IgY and non-immune IgY.

Scientific Data Images for Fibrillarin Antibody - BSA Free

Western Blot: Fibrillarin Antibody [NBP2-46881]

Western Blot: Fibrillarin Antibody [NBP2-46881]

Western Blot: Fibrillarin Antibody [NBP2-46881] - Western blot analysis of different cell lysates using chicken pAb to fibrillarin NBP2-46881, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, and [6] C6 cells. The single strong band at ~35kDa correspond to the fibrillarin protein.
Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody [NBP2-46881]

Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody [NBP2-46881]

Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP2-46881] - Confocal immunofluorescent analysis of HeLa cells stained with chicken pAb to fibrillarin, NBP2-46881, dilution 1:10,000 in green and costained with mouse mAb to vimentin, 1:1,000, in red. The blue signal is DAPI staining of nuclear DNA. The fibrillarin antibody stains nucleoli while the vimentin antibody binds to cytoplasmic intermediate filaments.

Applications for Fibrillarin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000-1:5000

Western Blot

1:2000-1:5000

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

Supplied as a concentrated total IgY preparation from egg yolk, dialyzed against PBS with added preservative.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibrillarin

Fibrillarin / Nop1p, was originally identified as a nucleolar protein of baker's yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is Fibrillarin (accession P22087), a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human Fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the Fibrillarin domain. A Fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout the entire Fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be Fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

Alternate Names

EC 2.1.1,34-kD nucleolar scleroderma antigen, EC 2.1.1.-, EC 2.1.1.37, FIB, FIB1,34 kDa nucleolar scleroderma antigen, fibrillarin, FLRNrRNA 2'-O-methyltransferase fibrillarin, RNA, U3 small nucleolar interacting protein 1, RNU3IP1

Entrez Gene IDs

2091 (Human)

Gene Symbol

FBL

UniProt

P22087 (Human)

Additional Fibrillarin Products

Product Documents for Fibrillarin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Fibrillarin Antibody - BSA Free

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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