FGFR1 Antibody (M17A3) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2079
![Flow Cytometry: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]](https://resources.bio-techne.com/images/products/FGF-R1-Antibody-M17A3-Flow-Cytometry-NB100-2079-img0005.jpg "Flow Cytometry: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]")
![Western Blot: FGFR1 Antibody (M17A3)BSA Free [NB100-2079]](https://resources.bio-techne.com/images/products/FGF-R1-Antibody-M17A3-Western-Blot-NB100-2079-img0004.jpg "Western Blot: FGFR1 Antibody (M17A3)BSA Free [NB100-2079]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # M17A3
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human ectodomain of FGF R1 expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
Epitope
Epitope is undetermined but presumed to be near junction of the common FGF R1 NH2-terminus and NH2-terminus of Ig loop II.
Localization
Cell Membrane
Specificity
Reacts with beta isoform of FRFR1. Western blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculoviral, or native FGFr1. Immunoprecipitation: native, nuclear, recombinant, and ligand-labeled FGFr1. Immunohistochemistry: under investigation.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for FGFR1 Antibody (M17A3) - BSA Free
Western Blot: FGFR1 Antibody (M17A3)BSA Free [NB100-2079]
Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-FGFR1 in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.![Immunohistochemistry-Paraffin: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]](https://resources.bio-techne.com/images/products/FGF-R1-Antibody-M17A3-Immunohistochemistry-Paraffin-NB100-2079-img0003.jpg "Immunohistochemistry-Paraffin: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]")
Immunohistochemistry-Paraffin: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]
Immunohistochemistry-Paraffin: FGF R1 Antibody (M17A3) [NB100-2079] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal skin using mouse monoclonal FGF R1 Antibody (clone M17A3) at 5 ug/ml concentration. The keratinocytes of the epidermal layer showed a very strong membrane-cytoplasmic with relatively weak nuclear staining for FGF R1 Antibody. The stratum basale layer of keratinocytes depicted more of membrane-cytoplasmic staining for this protein.![Flow Cytometry: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]](https://resources.bio-techne.com/images/products/FGF-R1-Antibody-M17A3-Flow-Cytometry-NB100-2079-img0006.jpg "Flow Cytometry: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]")
Flow Cytometry: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]
Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.Applications for FGFR1 Antibody (M17A3) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:500-1:1000
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Immunoprecipitation
1:10-1:500
Western Blot
2-4 ug/ml
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FGFR1
Long Name
Fibroblast Growth Factor Receptor 1
Alternate Names
CD331, FGF R1, Flt-2
Entrez Gene IDs
2260 (Human)
Gene Symbol
FGFR1
UniProt
Additional FGFR1 Products
Product Documents for FGFR1 Antibody (M17A3) - BSA Free
Product Specific Notices for FGFR1 Antibody (M17A3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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