I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?

Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Does the human beta-NGF polyclonal antibody (Catalog # AB-256-NA) cross-react with human BDNF, human NT-3, or mouse beta-NGF?

In Western blot, less than 5% cross-reactivity was observed with recombinant mouse β-NGF and recombinant rat β-NGF, and no cross-reactivity was observed with recombinant human (rh) NT-3 and rhNT-4.

What is the specificity and cross-reactivity of Catalog # AB-233-NA?

For Western blot, less than 5% cross-reactivity was observed with recombinant human (rh) β-ECGF, and no cross-reactivity was observed with rhFGF acidic, FGF-4, FGF-5, FGF-6, FGF-7, and FGF-9.

What is the heparin binding activity of FGF and VEGF?

These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.

What receptors does FGF basic bind?

FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.rndsystems.com/resources/articles/fibroblast-growth-factors-and-their-receptors

Does human FGF basic show activity on mouse cells?

Yes, it does. The bioassay uses NR-6 mouse fibroblast cells. There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.

FGF basic/FGF2/bFGF Antibody (8W6M5)

Name: FGF basic/FGF2/bFGF Antibody (8W6M5)
Brand: Novus Biologicals
SKU: NBP3-15367

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15367

Submit a review

Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8W6M5 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FGF basic/FGF2/bFGF Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human FGF basic/FGF2/bFGF (NP_001997.5). RAPERVGGRGRGRGTAAPRAAPAARGSRPGPAGTMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FGF basic/FGF2/bFGF Antibody (8W6M5)

Western Blot: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367]

Western Blot: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] - Analysis of extracts of SKOV3 cells, using FGF basic/FGF2/bFGF Rabbit mAb (NBP3-15367) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunocytochemistry/ Immunofluorescence: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367]

Immunocytochemistry/Immunofluorescence: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] - Immunofluorescence analysis of HeLa cells using FGF basic/FGF2/bFGF Rabbit mAb (NBP3-15367) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] -

Immunohistochemistry-Paraffin: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] -Analysis of FGF2 in paraffin-embedded human lung squamous carcinoma tissue using FGF2 Rabbit mAb at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

View 3 more images

Applications for FGF basic/FGF2/bFGF Antibody (8W6M5)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGF basic/FGF2/bFGF

FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Gene Symbol

FGF2

Additional FGF basic/FGF2/bFGF Products

Product Documents for FGF basic/FGF2/bFGF Antibody (8W6M5)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for FGF basic/FGF2/bFGF Antibody (8W6M5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

FGF basic/FGF2/bFGF Antibody (8W6M5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15367

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all FGF basic/FGF2/bFGF Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for FGF basic/FGF2/bFGF Antibody (8W6M5)

Western Blot: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367]

Immunocytochemistry/ Immunofluorescence: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367]

Immunohistochemistry-Paraffin: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] -

Immunohistochemistry-Paraffin: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] -

Immunohistochemistry-Paraffin: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] -

Immunohistochemistry-Paraffin: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] -

Applications for FGF basic/FGF2/bFGF Antibody (8W6M5)

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: FGF basic/FGF2/bFGF

Long Name

Alternate Names

Gene Symbol

Additional FGF basic/FGF2/bFGF Products

Product Documents for FGF basic/FGF2/bFGF Antibody (8W6M5)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for FGF basic/FGF2/bFGF Antibody (8W6M5)