FGF basic/FGF2/bFGF Antibody (24GB4420)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51547
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 24GB4420
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein of human FGF basic/FGF2/bFGF (Uniprot: P09038)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FGF basic/FGF2/bFGF Antibody (24GB4420)
Flow Cytometry: FGF basic/FGF2/bFGF Antibody (24GB4420)[NBP3-51547] -
Validation of FGF basic/FGF2/bFGF knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with FGF basic/FGF2/bFGF antibody (1:2,000) and analyzed using BD flow cytometer.
Flow Cytometry: FGF basic/FGF2/bFGF Antibody (24GB4420)[NBP3-51547] -
Flow cytometric analysis of FGF basic/FGF2/bFGFexpression in HepG2 cells using FGF basic/FGF2/bFGF antibody (1:2,000). Green, isotype control; red, FGF2.
Western Blot: FGF basic/FGF2/bFGF Antibody (24GB4420)[NBP3-51547] -
Western blotting analysis using FGF basic/FGF2/bFGF antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.Applications for FGF basic/FGF2/bFGF Antibody (24GB4420)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FGF basic/FGF2/bFGF
FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.
Long Name
Fibroblast Growth Factor basic
Alternate Names
bFGF, FGF-2, FGF2, HBGF-2, Prostatropin
Gene Symbol
FGF2
Additional FGF basic/FGF2/bFGF Products
Product Documents for FGF basic/FGF2/bFGF Antibody (24GB4420)
Product Specific Notices for FGF basic/FGF2/bFGF Antibody (24GB4420)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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