FEN-1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-321
![Western Blot: FEN-1 Antibody [NB100-321]](https://resources.bio-techne.com/images/products/FEN-1-Antibody-Western-Blot-NB100-321-img0012.jpg "Western Blot: FEN-1 Antibody [NB100-321]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Flap Structure Endonuclease 1 using the numbering given in entry NP_004102.1 (GeneID 2237).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FEN-1 Antibody - BSA Free
Western Blot: FEN-1 Antibody [NB100-321]
Western Blot: FEN-1 Antibody [NB100-321] - Detection of Human Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody NB100-321 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321]](https://resources.bio-techne.com/images/products/FEN-1-Antibody-Immunohistochemistry-NB100-321-img0013.jpg "Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321]")
Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321]
Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321] - FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Fen1 (NB100-321) used at a dilution of 1:1000 (1ug/ml). Detection: DAB![Immunoprecipitation: FEN-1 Antibody [NB100-321]](https://resources.bio-techne.com/images/products/FEN-1-Antibody-Immunoprecipitation-NB100-321-img0014.jpg "Immunoprecipitation: FEN-1 Antibody [NB100-321]")
Immunoprecipitation: FEN-1 Antibody [NB100-321]
Immunoprecipitation: FEN-1 Antibody [NB100-321] - Detection of human Fen1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody NB100-321 (lot NB100-321-2) used for IP at 6 ug per reaction. Fen1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-321-1). For blotting immunoprecipitated Fen1, NB100-321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for FEN-1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:200- 1:1000
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. 16-18 hour incubation is recommended
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: FEN-1
Long Name
Flap Endonuclease 1
Alternate Names
FEN1
Entrez Gene IDs
2237 (Human)
Gene Symbol
FEN1
UniProt
Additional FEN-1 Products
Product Documents for FEN-1 Antibody - BSA Free
Product Specific Notices for FEN-1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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