Skip to main content

Key Product Details

Species Reactivity

Feline

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant feline CD8α
Ala22-Tyr188
Accession # P41688

Specificity

Detects feline CD8 alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse CD8 alpha is observed, approximately 5% cross-reactivity with recombinant canine CD8 alpha is observed, a

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Feline CD8 alpha Alexa Fluor® 532-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CD8

CD8 is a dimeric complex made up of two Ig superfamily members. The CD8 alpha chain is a 34 kDa type I transmembrane glycoprotein that is disulfide-linked, either to itself forming CD8 alpha alpha, or to an unrelated 30-35 kDa CD8 beta chain, forming CD8 alpha beta. CD8 alpha contains one V-type Ig-like domain in its extracellular region that binds to class I MHC molecules. CD8 alpha beta is a TCR coreceptor, while CD8 alpha alpha promotes T cell survival and differentation. Feline CD8 alpha extracellular region shares 58%, 47%, 51%, and 69% amino acid sequence identity with human, mouse, porcine, and canine CD8 alpha extracellular regions, respectively.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

Additional CD8 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Feline CD8 alpha Alexa Fluor® 532-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Loading...
Loading...
Loading...