FcgR4/CD16-2 Antibody (012) [DyLight 350]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05890UV
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
DyLight 350 (Excitation = 353 nm, Emission = 432 nm)
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 012 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FcgR4/CD16-2 (Accession#: NP_653142.2; Met 1-Gln 203).
Reactivity Notes
Has cross-reactivity in ELISA with:
Human CD64 / FCGR1A
No cross-reactivity in ELISA with:
Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3
Human CD64 / FCGR1A
No cross-reactivity in ELISA with:
Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3
Specificity
Has cross-reactivity in ELISA with:
Human CD64 / FCGR1ANo cross-reactivity in ELISA with:
Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Applications for FcgR4/CD16-2 Antibody (012) [DyLight 350]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: FcgR4/CD16-2
Fcgr4, also known as Fc Receptor-like 3 (Fcrl3) and CD16-2, is a recently described mouse protein that is closely related to human Fc gamma RIIIA (60% amino acid identity) and may be its true ortholog. Similar to other activating receptors, it possesses a charged residue within its transmembrane domain that is necessary for association with an ITAM bearing accessory protein. CD16-2 mRNA expression was observed in peripheral blood leukocytes, spleen, thymus, colon, and intestine.
Long Name
Fc receptor, IgG, low affinity IV
Alternate Names
CD16-2, Fcrl3
Gene Symbol
Fcgr4
Additional FcgR4/CD16-2 Products
Product Documents for FcgR4/CD16-2 Antibody (012) [DyLight 350]
Product Specific Notices for FcgR4/CD16-2 Antibody (012) [DyLight 350]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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