Fc gamma RIIB/C (CD32b/c) Antibody (190710) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB18751CL1
![Fc gamma RIIB/C (CD32b/c) Antibody (190710) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/fab18751cl1_mouse-fc-gamma-riib-c-cd32b-c-mab-190710-corafluor-1-311220251242923.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair)
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG2B Clone # 190710
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIB/C
Ala46-Pro217
Accession # P31994
Ala46-Pro217
Accession # P31994
Specificity
Detects human Fc gamma RIIB/C in ELISAs. In a sandwich ELISA shows approximately 40% cross-reactivity with recombinant human (rh) Fc gamma RIIA and no cross‑reactivity with rhFc gamma RI, rmFc gamma RI, rmFc gamma RIIB, rhFc gamma RIIIB, rmFc gamma RIII, or rmFcrl3/CD16-2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for Fc gamma RIIB/C (CD32b/c) Antibody (190710) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for Fc gamma RIIB/C (CD32b/c) Antibody (190710) [CoraFluor™ 1]
Application
Recommended Usage
ELISA Capture (Matched Antibody Pair)
Optimal dilutions of this antibody should be experimentally determined.
ELISA Detection (Matched Antibody Pair)
Optimal dilutions of this antibody should be experimentally determined.
ELISA Standard (Matched Pair)
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Fc gamma RIIB/C (CD32b/c)
Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses. CD32 proteins bind IgG with lower affinities and recognize IgG aggregates surrounding multivalent antigens. They are expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Fc gamma RIIA associates with FcR gamma and delivers an activating signal through its cytoplasmic ITAM motif. In contrast, Fc gamma RIIB delivers an inhibitory signal through its ITIM motif. Fc gamma RIIC represents an unequal cross-over event between the IIA and IIB genes.
Long Name
Fc gamma Receptor IIB/C
Alternate Names
CD32;CD32B;FCG2;FcgammaRIIb;FCGR2;FCGR2B;FCGR2C;FcGRIIB;FcRII-c;IGFR2;Low affinity immunoglobulin gamma Fc region receptor II-b
Gene Symbol
FCGR2B
Additional Fc gamma RIIB/C (CD32b/c) Products
Product Documents for Fc gamma RIIB/C (CD32b/c) Antibody (190710) [CoraFluor™ 1]
Product Specific Notices for Fc gamma RIIB/C (CD32b/c) Antibody (190710) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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