Fc gamma RII/RIII (CD32/CD16) Antibody (190909) [Janelia Fluor® 646]
Novus Biologicals, part of Bio-Techne | Catalog # FAB1460J
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Janelia Fluor 646
Antibody Source
Recombinant Monoclonal Rat IgG2B Clone # 190909
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
Thr30-Arg207
Accession # P08101
Thr30-Arg207
Accession # P08101
Reactivity Notes
0
Specificity
Detects mouse Fc gamma RII/RIII (CD32/CD16) in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse CD16 is observed and no cross-reactivity with recombinant human CD32 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for Fc gamma RII/RIII (CD32/CD16) Antibody (190909) [Janelia Fluor® 646]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Fc gamma RII/RIII (CD32/CD16)
Fc gamma RII/CD32 and Fc gamma RIII/CD16 proteins are low/intermediate affinity receptors for polyvalent immune-complexed IgG. Fc gamma RII is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Fc gamma RIIA associates with FcR gamma and delivers an activating signal through its cytoplasmic ITAM motif. In contrast, Fc gamma RIIB delivers an inhibitory signal through its ITIM motif. Fc gamma RIIC represents an unequal cross-over event between the IIA and IIB genes. Fc gamma RIII is expressed on NK cells, T cells, monocytes, and macrophages. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes.
Long Name
Fc gamma Receptor II/III
Alternate Names
CD32/CD16
Additional Fc gamma RII/RIII (CD32/CD16) Products
Product Specific Notices for Fc gamma RII/RIII (CD32/CD16) Antibody (190909) [Janelia Fluor® 646]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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