FACL4 Antibody

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401]

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - Rat middle brain. FACL4 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401]

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - Mouse lymph node. FACL4 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation: FACL4 Antibody [NBP2-16401]

Immunoprecipitation: FACL4 Antibody [NBP2-16401] - Immunoprecipitation of FACL4 protein from HeLa whole cell extracts using 5 ug of FACL4 antibody [C3], C-term Western blot analysis was performed using FACL4 antibody [C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] -

FACL4 antibody [C3], C-term detects FACL4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: FACL4 Antibody [NBP2-16401] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: FACL4 Antibody [NBP2-16401] -

FACL4 antibody [C3], C-term detects FACL4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI .

Western Blot: FACL4 Antibody [NBP2-16401] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] -

FACL4 antibody [C3], C-term detects FACL4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: FACL4 Antibody [NBP2-16401] -

Expression and location of ferroptosis-related proteins in diabetic mice retinas. (A) Western blot analysis of ferroptosis-related proteins expression levels in retinas at 1 and 3 months post-diabetes. GAPDH was used as a control. (B) GPX4 and SLC7A11 protein expression was remarkably decreased in diabetic mice retinas compared with the control retinas. Expression of ACSL4, FTH1, and NCOA4 proteins was significantly increased in retinas at 1 and 3 months post-diabetes. (C) Immunofluorescence staining of location of ferroptosis-related proteins (red) and nuclear (blue) in retinas at 1 and 3 months post-diabetes: ganglion cell layer (GCL), inner nuclear layer (INL), outer nuclear layer (ONL), inner segment (IS), outer segment (OS). Data are shown as mean +/- SEM, n = 3 per group for Western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FACL4 Antibody [NBP2-16401] -

HG causes changes in the expression of ferroptosis-related proteins in 661W cells. (A) Western blot analysis of ferroptosis-related protein expression levels in HG-induced 661W cells. GAPDH was used as a control. (B) Expression of GPX4 and SLC7A11 proteins was significantly downregulated in HG-stimulated 661W cells after 12, 18, and 24 h. HG induced obvious upregulation in the expression of ACSL4, FTH1, and NCOA4 in 661W cells compared with the Ctrl group. (C) Immunofluorescence staining of localization of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after 18 h. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01, *** p < 0.001 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FACL4 Antibody [NBP2-16401] -

Fer-1 treatment attenuated changes in ferroptosis-related proteins’ expression in HG-stimulated 661W cells after 18 h. (A) Western blot analysis of ferroptosis-related proteins’ expression levels in HG-induced 661W cells after Fer-1 treatment. GAPDH was used as a control. (B) The downregulation in GPX4 and SLC7A11 protein expression in HG-stimulated 661W cells was significantly attenuated after Fer-1 treatment. The upregulation in ACSL4, FTH1, and NCOA4 protein expression in HG-stimulated 661W cells was effectively abrogated after Fer-1 treatment. (C) Immunofluorescence staining of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after Fer-1 administration. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FACL4 Antibody [NBP2-16401] -

Fer-1 administration attenuated changes of ferroptosis-related proteins expression in diabetic mice retinas. (A) Western blot analysis of ferroptosis-related proteins expression levels in diabetic mice retinas after Fer-1 treatment. GAPDH was used as a control. (B) The decrease in GPX4 and SLC7A11 protein expression in diabetic mice retinas was significantly attenuated after Fer-1 treatment. The increase in ACSL4, FTH1, and NCOA4 protein expression in diabetic mice retinas was effectively abrogated after Fer-1 treatment. (C) Immunofluorescence staining of ferroptosis-related proteins (red) and nuclear (blue) in diabetic mice retinas after Fer-1 treatment: ganglion cell layer (GCL), inner nuclear layer (INL), outer nuclear layer (ONL), inner segment (IS), outer segment (OS). Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FACL4 Antibody [NBP2-16401] -

Extraction ion-containing PMs (PM2.5 EI) cause ferroptosis in macrophages.A Western blot analysis after incubation of RAW264.7 cells with three types of PM (100 μg/ml) for 18 h. B Malondialdehyde (MDA) formation in RAW264.7 cells after incubation with 100 μg/ml PMs for 12 h, investigated using a lipid peroxidation assay kit. C Intracellular ferrous iron levels are detected in J774A.1 cells incubated with 50 μg/ml of PMs for 12 h. D WST-8 assay demonstrating the cell viability analysis in RAW264.7 line after pretreatment with ferrostatin-1 (Fer-1; 2 μM), liproxstatin-1 (Lip-1; 2 μM), or deferiprone (DFP; 100 μM) for 2 h followed by the stimulation with 100 μg/ml of PM2.5 EI for 24 h. E Western blot analysis using RAW264.7 cells after preincubation with Fer-1 (2 μM), Lip-1 (2 μM), or DFP (100 μM) for 2 h followed by stimulation with 100 μg/ml of PM2.5 EI for 24 h. F Detection of lipid peroxidation in terms of MDA in J774A.1 cells after preincubation with Fer-1 (2 μM), Lip-1 (2 μM), or DFP (100 μM) for 2 h followed by treatment using 50 μg/ml of PM2.5 EI for 12 h. All data are presented as the means +/- standard deviations from at least three independent experiments. *P < 0.05, **P < 0.01 and #P < 0.001. All experiments were conducted at least three times. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41420-024-01874-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FACL4 Antibody [NBP2-16401] -

PM2.5 EI induces a correlation between the activation of inflammasomes and the induction of ferroptosis.A and B Western blot analysis in J774A.1 cells after preincubation with CY-09 (10 μM) or YVAD (20 μM) for 2 h and subsequent stimulation with 50 μg/ml of PM2.5 EI for 24 h. C MDA levels in J774A.1 cells after pretreatment with CY-09 (10 μM) or YVAD (20 μM) for 2 h followed by stimulation with 50 μg/ml of PM2.5 EI for 12 h tested using a lipid peroxidation assay kit. D Western blot analysis using J774A.1 cells preincubated with Fer-1 (2 μM), Lip-1 (2 μM), or DFP (100 μM) for 2 h and treated using 50 μg/ml of PM2.5 EI for 24 h. E, F ELISA assay revealed the IL-1 beta and IL-18 levels in the medium of J774A.1 cell culture after pretreatment with CY-09 (10 μM) or YVAD (20 μM) for 2 h and subsequent stimulation with 50 μg/ml of PM2.5 EI for 12 h. All data are presented as the means +/- standard deviations from at least three independent experiments. *P < 0.05, **P < 0.01 and #P < 0.001. All experiments were conducted at least three times. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41420-024-01874-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: FACL4 Antibody [NBP2-16401] -

HG causes changes in the expression of ferroptosis-related proteins in 661W cells. (A) Western blot analysis of ferroptosis-related protein expression levels in HG-induced 661W cells. GAPDH was used as a control. (B) Expression of GPX4 and SLC7A11 proteins was significantly downregulated in HG-stimulated 661W cells after 12, 18, and 24 h. HG induced obvious upregulation in the expression of ACSL4, FTH1, and NCOA4 in 661W cells compared with the Ctrl group. (C) Immunofluorescence staining of localization of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after 18 h. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01, *** p < 0.001 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.