F4/80 Antibody (Cl:A3-1 (recombinant version)) [Alexa Fluor™ Plus 647]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81030AFP647

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Flow Cytometry

Label

Alexa Fluor Plus 647

Antibody Source

Recombinant Monoclonal Rat IgG2b Kappa Clone # Cl:A3-1 (recombinant version)

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all F4/80 Antibodies »

Product Specifications

Immunogen

This F4/80 Antibody (Cl:A3-1 (recombinant version)) was developed against Thioglycollate stimulated peritoneal macrophages of mouse origin.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Applications

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: F4/80

F4/80 antigen is a cell surface glycoprotein that is the murine homolog of human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) (1,2). Specifically, F4/80 (EMR1) is one of five members belonging to the adhesion G-coupled receptor (ADGRE) subfamily, also known as the epidermal growth factor seven transmembrane (EGF-TM7) receptors (1,2). F4/80 is expressed by the myeloid lineage including in eosinophils, monocytes, macrophages, and myeloid dendritic cells (1,2). The molecular structure of F4/80 contains an EGF-like extracellular domain and a seven transmembrane motif, with a theoretical molecular weight of 160 kDa (1).

Depending on factors such as differentiation, activation, and location, the level of F4/80 expression can vary between cell-types (1,2). For example, peritoneal macrophages are subdivided based on morphology and expression with large peritoneal macrophages having high levels of F4/80 and small peritoneal macrophages having low F4/80 expression (1). Furthermore, splenic macrophages are classified based on location (red pulp, marginal zone, and white pulp) and F4/80 expression level varies within these populations as well; the red pulp macrophages have high levels of F4/80 and CD68 by comparison (1). Originally, the F4/80 monoclonal antibody was generated for a macrophage-specific surface antigen, however F4/80 staining is also observed in other hematopoietic cell populations, thus other immune cell markers should be used in conjunction with F4/80 to distinguish between different populations (1). Though the full extent of F4/80 ligands and biological functions remains elusive, studies have suggested that F4/80 is critical for macrophage-natural killer cell interaction and cytokine release (1,2). Additionally, a study of anterior chamber-associated immune deviation (ACAID) indicated a possible role for F4/80 in the generation of regulatory T cells (1,2).

References

1. Dos Anjos Cassado A. (2017). F4/80 as a Major Macrophage Marker: The Case of the Peritoneum and Spleen. Results and Problems in Cell Differentiation. https://doi.org/10.1007/978-3-319-54090-0_7

2. Hamann, J., Hsiao, C. C., Lee, C. S., Ravichandran, K. S., & Lin, H. H. (2016). Adhesion GPCRs as Modulators of Immune Cell Function. Handbook of Experimental Pharmacology. https://doi.org/10.1007/978-3-319-41523-9_15

Alternate Names

DD7A5-7, Gpf480, Ly71

Gene Symbol

Adgre1

Additional F4/80 Products

Product Documents

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Certificate of Analysis

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Product Specific Notices



This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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