ERG Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2472
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-PNTRLPTSHMPSH corresponding to C-Terminus according to NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1|.
Epitope
C-PNTRLPTSHMPSH
Specificity
This antibody is expected to recognise all reported isoforms (NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1)
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Novus Biologicals Goat ERG Antibody (NB100-2472) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-ERG Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ERG Antibody
![Western Blot: ERG Antibody [NB100-2472]](https://resources.bio-techne.com/images/products/ERG-Antibody-Western-Blot-NB100-2472-img0001.jpg "Western Blot: ERG Antibody [NB100-2472]")
Western Blot: ERG Antibody [NB100-2472]
Western Blot: ERG Antibody [NB100-2472] - Staining of MOLT4 lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: ERG Antibody [NB100-2472]](https://resources.bio-techne.com/images/products/ERG-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2472-img0002.jpg "Immunocytochemistry/ Immunofluorescence: ERG Antibody [NB100-2472]")
Immunocytochemistry/ Immunofluorescence: ERG Antibody [NB100-2472]
Immunocytochemistry/Immunofluorescence: ERG Antibody [NB100-2472] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).![Flow Cytometry: ERG Antibody [NB100-2472]](https://resources.bio-techne.com/images/products/ERG-Antibody-Flow-Cytometry-NB100-2472-img0003.jpg "Flow Cytometry: ERG Antibody [NB100-2472]")
Flow Cytometry: ERG Antibody [NB100-2472]
Flow Cytometry: ERG Antibody [NB100-2472] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for ERG Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:6000
Western Blot
0.1 - 0.3 ug/mL
Reviewed Applications
Read 1 review rated 1 using NB100-2472 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Erg
Long Name
v-Ets Erythroblastosis Virus E26 Oncogene Homolog
Alternate Names
Erg-3, p55
Gene Symbol
ERG
UniProt
Additional Erg Products
Product Documents for ERG Antibody
Product Specific Notices for ERG Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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