ER alpha/NR3A1 Antibody (CL1196) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34478

Novus Biologicals, part of Bio-Techne
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NBP2-34478-25ul
NBP2-34478
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Conjugate
Catalog #
Alexa Fluor 350
NBP3-43926AF350
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Alexa Fluor 405
NBP3-43926AF405
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Alexa Fluor 488
NBP3-43926AF488
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Alexa Fluor 532
NBP3-43926AF532
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Alexa Fluor 594
NBP3-43926AF594
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Alexa Fluor 647
NBP3-43926AF647
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Alexa Fluor 700
NBP3-43926AF700
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Alexa Fluor 750
NBP3-43926AF750
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Alexa Fluor Plus 488
NBP3-43926AFP488
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Alexa Fluor Plus 555
NBP3-43926AFP555
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Alexa Fluor Plus 594
NBP3-43926AFP594
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Alexa Fluor Plus 647
NBP3-43926AFP647
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Alexa Fluor Plus 680
NBP3-43926AFP680
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Allophycocyanin
NBP3-43926APC
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Biotin
NBP3-43926B
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CoraFluor 1
NBP3-43926CL1
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DyLight 350
NBP3-43926UV
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DyLight 405
NBP3-43926V
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DyLight 488
NBP3-43926G
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DyLight 550
NBP3-43926R
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DyLight 594
NBP3-43926DL594
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DyLight 650
NBP3-43926C
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DyLight 680
NBP3-43926FR
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DyLight 755
NBP3-43926IR
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FITC
NBP3-43926F
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HRP
NBP3-43926H
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Janelia Fluor 525
NBP3-43926JF525
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Janelia Fluor 549
NBP3-43926JF549
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Janelia Fluor 646
NBP3-43926JF646
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Janelia Fluor 669
NBP3-43926JF669
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mFluor Violet 450 SE
NBP3-43926MFV450
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mFluor Violet 500 SE
NBP3-43926MFV500
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mFluor Violet 610 SE
NBP3-43926MFV610
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PE
NBP3-43926PE
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PerCP
NBP3-43926PCP
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1196

Format

BSA Free

Concentration

1 mg/ml

View all ER alpha/NR3A1 Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P03372, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ER alpha/NR3A1 Antibody (CL1196) - BSA Free

Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of MCF7 cells using the Anti-ESR1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human testis shows absence of immunoreactivity (negative control).
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Applications for ER alpha/NR3A1 Antibody (CL1196) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ER alpha/NR3A1

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Gene Symbol

ESR1

UniProt

P03372

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (CL1196) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ER alpha/NR3A1 Antibody (CL1196) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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