epithelial Sodium Channel gamma Antibody (5c2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41373
![Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373]](https://resources.bio-techne.com/images/products/epithelial-Sodium-Channel-gamma-Antibody-5c2-BSA-Free-Immunohistochemistry-Paraffin-NBP2-41373-img0003.jpg "Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5c2
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to
amino acid residue 139-160 of human yENaC subunit.
Epitope
The epitope is on the inhibitory tract of human yENaC.
Specificity
Antibody is specific for the inhibitory tract of human gENaC.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for epithelial Sodium Channel gamma Antibody (5c2) - BSA Free
Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373]
Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373] - Analysis using the Biotin conjugate of NBP2-41373. Staining of Human kidney tissue. Courtesy of Per Svenningsen, PhD.
Sandwich ELISA: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373] - Analysis using the Biotin conjugate of NBP2-41373. The calibration curveof a sandwich assay using NBP2-41371 as the capture antibody and NBP2-41372B as the biotinylated detection antibody.
Applications for epithelial Sodium Channel gamma Antibody (5c2) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
ELISA: A sandwich ELISA can be made using NBP2-41371 (1-4 ug/ml) as the capture antibody and biotinylated NBP2-41373 (0.05 - 0.2 ug/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131).
WB: NBP2-41373 was used in Western blot. (1)
IHC: NBP2-41373 was used in immunohistochemistry. (1)
IF: NBP2-41373 was used in immunofluorescence. (1)
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: epithelial Sodium Channel gamma
Long Name
Amiloride-sensitive sodium channel subunit gamma
Alternate Names
ENaCG, Gamma-ENaC, Gamma-NaCH, SCNEG, SCNN1G
Gene Symbol
SCNN1G
Additional epithelial Sodium Channel gamma Products
Product Documents for epithelial Sodium Channel gamma Antibody (5c2) - BSA Free
Product Specific Notices for epithelial Sodium Channel gamma Antibody (5c2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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