epithelial Sodium Channel gamma Antibody (3c7) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41371
![Western Blot: epithelial Sodium Channel gamma Antibody (3c7)BSA Free [NBP2-41371]](https://resources.bio-techne.com/images/products/epithelial-Sodium-Channel-gamma-Antibody-3c7-BSA-Free-Western-Blot-NBP2-41371-img0006.jpg "Western Blot: epithelial Sodium Channel gamma Antibody (3c7)BSA Free [NBP2-41371]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Sandwich ELISA, Sandwich ELISA Capture
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3c7
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The inhibitory peptide from the human gamma-ENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human gamma-ENaC subunit.
Epitope
The epitope is on the inhibitory tract of human ENaC.
Specificity
Antibody is specific for the inhibitory tract of human gamma-ENaC subunit.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Concentration for any given batch will be between 0.85 - 1.15 mg/mL.
Scientific Data Images for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free
Western Blot: epithelial Sodium Channel gamma Antibody (3c7)BSA Free [NBP2-41371]
Western Blot: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371] - Analysis on a lysate of HEK293 cells transfected with and without yENaC. Lane 1 loaded with 5ug Lane 2 loaded with 10ug Lane 3 loaded with 20ug![ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371]](https://resources.bio-techne.com/images/products/epithelial-Sodium-Channel-gamma-Antibody-3c7-BSA-Free-ELISA-NBP2-41371-img0007.jpg "ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371]")
ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371]
ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371] - The calibration curve of a sandwich assay for Anti-gENaC using NBP2-41371 as the capture antibody and NBP2-41373B as the biotinylated detection antibody.Applications for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Sandwich ELISA Capture
1-4 ug/ml
Western Blot
1:100-1:2000
Application Notes
ELISA: NBP2-41371 was used in ELISA. A sandwich ELISA can be made using NBP2-41371 (1-4 ug/ml) as the capture antibody and biotinylated NBP2-41373 (0.05 - 0.2 ug/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131). WB: NBP2-41371 was used in Western blot. (1,2) IHC: NBP2-41371 was used in immunohistochemistry.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: epithelial Sodium Channel gamma
Long Name
Amiloride-sensitive sodium channel subunit gamma
Alternate Names
ENaCG, Gamma-ENaC, Gamma-NaCH, SCNEG, SCNN1G
Gene Symbol
SCNN1G
Additional epithelial Sodium Channel gamma Products
Product Documents for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free
Product Specific Notices for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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