Ephrin-B2 Antibody (1G9E7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15372
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1G9E7 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EFNB2 (P52799). MAVRRDSVWKYCWGVLMVLCRTAISKSIVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIKKENTPLLN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Ephrin-B2 Antibody (1G9E7) (NBP3-15372) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Ephrin-B2 Antibody (1G9E7)
![Western Blot: Ephrin-B2 Antibody (1G9E7) [NBP3-15372]](https://resources.bio-techne.com/images/products/Ephrin-B2-Antibody-1G9E7-Western-Blot-NBP3-15372-img0002.jpg "Western Blot: Ephrin-B2 Antibody (1G9E7) [NBP3-15372]")
Western Blot: Ephrin-B2 Antibody (1G9E7) [NBP3-15372]
Western Blot: Ephrin-B2 Antibody (1G9E7) [NBP3-15372] - Western blot analysis of extracts of various cell lines, using Ephrin-B2 Rabbit mAb (NBP3-15372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody (1G9E7) [NBP3-15372]](https://resources.bio-techne.com/images/products/Ephrin-B2-Antibody-1G9E7-Immunohistochemistry-Paraffin-NBP3-15372-img0001.jpg "Immunohistochemistry-Paraffin: Ephrin-B2 Antibody (1G9E7) [NBP3-15372]")
Immunohistochemistry-Paraffin: Ephrin-B2 Antibody (1G9E7) [NBP3-15372]
Immunohistochemistry-Paraffin: Ephrin-B2 Antibody (1G9E7) [NBP3-15372] - Immunohistochemistry of paraffin-embedded rat heart using Ephrin-B2 Rabbit mAb (NBP3-15372) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Western Blot: Ephrin-B2 Antibody (1G9E7) [NBP3-15372]](https://resources.bio-techne.com/images/products/Ephrin-B2-Antibody-1G9E7-Western-Blot-NBP3-15372-img0003.jpg "Western Blot: Ephrin-B2 Antibody (1G9E7) [NBP3-15372]")
Western Blot: Ephrin-B2 Antibody (1G9E7) [NBP3-15372]
Western Blot: Ephrin-B2 Antibody (1G9E7) [NBP3-15372] - Western blot analysis of extracts of various cell lines, using Ephrin-B2 Rabbit mAb (NBP3-15372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Applications for Ephrin-B2 Antibody (1G9E7)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ephrin-B2
Ephrins are naturally divided into two structural groups. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins, which can be tyrosine phosphorylated upon receptor ligation. Class B ephrins show 33% amino acid sequence identity in their extracellular segments and 44% amino acid sequence identity in their cytoplasmic regions.
Alternate Names
EFNB2, ELF-2, EphrinB2, Htk-L, LERK-5, NLERK-1
Gene Symbol
EFNB2
Additional Ephrin-B2 Products
Product Documents for Ephrin-B2 Antibody (1G9E7)
Product Specific Notices for Ephrin-B2 Antibody (1G9E7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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