EN1/Engrailed 1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13332

Novus Biologicals, part of Bio-Techne
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NBP3-13332
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Engrailed-1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse EN1/Engrailed 1. The exact sequence is proprietary.

Reactivity Notes

Human (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for EN1/Engrailed 1 Antibody - BSA Free

Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody [NBP3-13332]

Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody [NBP3-13332]

Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody [NBP3-13332] - Mouse spinal cord. EN1/Engrailed 1 stained by EN1/Engrailed 1 antibody (NBP3-13332) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody [NBP3-13332]

Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody [NBP3-13332]

Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody [NBP3-13332] - Mouse brain. EN1/Engrailed 1 stained by EN1/Engrailed 1 antibody (NBP3-13332) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Frozen: EN1/Engrailed 1 Antibody [NBP3-13332]

Immunohistochemistry-Frozen: EN1/Engrailed 1 Antibody [NBP3-13332]

Immunohistochemistry-Frozen: EN1/Engrailed 1 Antibody [NBP3-13332] - EN1/Engrailed 1 antibody detects EN1/Engrailed 1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: EN1/Engrailed 1 stained by EN1/Engrailed 1 antibody (NBP3-13332) diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.

Applications for EN1/Engrailed 1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Engrailed-1

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Alternate Names

EN1, Engrailed1

Gene Symbol

EN1

Additional Engrailed-1 Products

Product Documents for EN1/Engrailed 1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EN1/Engrailed 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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