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ELAVL2 Antibody (CL15323) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44321

Novus Biologicals, part of Bio-Techne
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NBP3-44321
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # CL15323

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q12926, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for ELAVL2 Antibody (CL15323) - Azide and BSA Free

ELAVL2 Antibody (CL15323) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: ELAVL2 Antibody [NBP3-44321]

Immunocytochemistry/ Immunofluorescence: ELAVL2 Antibody [NBP3-44321]

Staining in A-431 cell line with Anti-ELAVL2 monoclonal antibody) .
ELAVL2 Antibody (CL15323) - Azide and BSA Free Immunohistochemistry-Paraffin: ELAVL2 Antibody [NBP3-44321]

Immunohistochemistry-Paraffin: ELAVL2 Antibody [NBP3-44321]

Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-44321 antibody. Corresponding ELAVL2 RNA-seq data are presented for the same tissues.
ELAVL2 Antibody (CL15323) - Azide and BSA Free Immunohistochemistry-Paraffin: ELAVL2 Antibody [NBP3-44321]

Immunohistochemistry-Paraffin: ELAVL2 Antibody [NBP3-44321]

Staining of human cerebellum shows strong cytoplasmic positivity in a subset of neurons in the granular layer.

Applications for ELAVL2 Antibody (CL15323) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000-1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ELAVL2

Hu-antigen B (HuB), ELAV-like protein 2, ELAV-like neuronal protein 1, Nervous system-specific RNA binding protein Hel-N1 is a transcription factor belonging to the ELAV family of RNP proteins. It binds RNA and seems to recognize a GAAA motif and is neuronal specific.

Alternate Names

ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B), ELAV-like neuronal protein 1, ELAV-like protein 2, HELN1, HEL-N1, Hu-antigen B, HuB, HUBELAV (embryonic lethal, abnormal vision, Drosophila)-like 2, Nervous system-specific RNA-binding protein Hel-N1

Gene Symbol

ELAVL2

Additional ELAVL2 Products

Product Documents for ELAVL2 Antibody (CL15323) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ELAVL2 Antibody (CL15323) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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