Elastin Antibody (MSVA-648R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-57257
Recombinant Monoclonal Antibody
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # MSVA-648R
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full-length human Elastin protein Swissprot P15502
Localization
Extracellular
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is highly specific and optimized for use in Immunohistochemistry.
It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.
The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.
Positive Control
Postive control:Colon: A moderate to strong elastin immunostaining should be seen of fibres in the lamina propria, around smooth muscle cells of the muscularis mucosae, and in blood vessels (subendothelial)..
Negative Control
Negitve control:Colon: Elastin immunostaining should be absent in all epithelial cells.
Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Elastin Antibody (MSVA-648R)
Immunohistochemistry-Paraffin:Elastin Antibody (MSVA-648R)NBP3-57257
Testis High density of elastin fibres along smooth muscles surrounding tubuli of the testis.
Immunohistochemistry-Paraffin:Elastin Antibody (MSVA-648R)NBP3-57257
Bronchus mucosa High content of elastin fibres along the bronchus mucosa.
Immunohistochemistry-Paraffin:Elastin Antibody (MSVA-648R)NBP3-57257
Colon descendes mucosa Elastin fibres predominate in the lamina propria around smooth muscle cells of the muscularis mucosae and in blood vessels.
Applications for Elastin Antibody (MSVA-648R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Preservative
<0.1% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Elastin
Alternate Names
elastin, FLJ38671, FLJ43523, SVAS, tropoelastin, WBS, WS
Gene Symbol
ELN
Additional Elastin Products
Product Documents for Elastin Antibody (MSVA-648R)
Product Specific Notices for Elastin Antibody (MSVA-648R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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