eIF5A2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85943
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IKRNDYQLICIQDGYLSLLTETGEVREDLKLPEGELGKEIEGKYNAGEDVQVSVMCAMSEEYAVA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for eIF5A2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: eIF5A2 Antibody [NBP1-85943]
Immunocytochemistry/Immunofluorescence: eIF5A2 Antibody [NBP1-85943] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943]
Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943] - Staining of human kidney shows moderate positivity in nuclear membrane in cells in tubules.Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943]
Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943] - Staining of human testis shows strong positivity in nuclear membrane in Leydig cells and a subset of cells in seminiferous ducts.Applications for eIF5A2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: eIF5A2
Alternate Names
EIF-5A2, eIF-5A-2, eIF5AII, eukaryotic initiation factor 5A, Eukaryotic initiation factor 5A isoform 2, eukaryotic translation initiation factor 5A2, eukaryotic translation initiation factor 5A-2
Gene Symbol
EIF5A2
Additional eIF5A2 Products
Product Documents for eIF5A2 Antibody - BSA Free
Product Specific Notices for eIF5A2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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