Skip to main content

EGLN3/PHD3 Antibody [DyLight 594]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-303DL594

Catalog #
Availability
Size / Price
Qty
Loading...
NB100-303DL594
Forumulation
Catalog #

Key Product Details

Species Reactivity

Human, Mouse, Rat, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

DyLight 594 (Excitation = 593 nm, Emission = 618 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide made to the C-terminus of humanPHD3/HIF Prolyl Hydroxylase 3. [LocusLink ID 112399]

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 23732909). Rat reactivity reported in scientific literature (PMID: 23545307).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for EGLN3/PHD3 Antibody [DyLight 594]

Application
Recommended Usage

Chromatin Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB100-303. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: EGLN3/PHD3

HIF prolyl hydroxylase 3 (HPH-3; PHD3; EGLN3) catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. PHD3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. EGLN3 may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. HIF prolyl hydroxylase 3 promotes cell death through a caspase-dependent mechanism.

Long Name

Egl Nine Homolog 3/Prolyl Hydroxylase Domain-containing Protein 3

Alternate Names

HIFPH3, PHD3

Gene Symbol

EGLN3

Additional EGLN3/PHD3 Products

Product Documents for EGLN3/PHD3 Antibody [DyLight 594]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for EGLN3/PHD3 Antibody [DyLight 594]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...