EEF2K Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-87019
![Western Blot: EEF2K Antibody [NB100-87019]](https://resources.bio-techne.com/images/products/EEF2K-Antibody-Western-Blot-NB100-87019-img0002.jpg "Western Blot: EEF2K Antibody [NB100-87019]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 675 and 725 of human calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase (EEF2K ) using the numbering given in entry NP_037434.1 (GeneID 29904).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EEF2K Antibody - BSA Free
Western Blot: EEF2K Antibody [NB100-87019]
Western Blot: EEF2K Antibody [NB100-87019] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-87019 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eEF2 Kinase was successfully immunoprecipitated by NB100-87018, which recognizes an upstream epitope.![Immunohistochemistry-Paraffin: EEF2K Antibody [NB100-87019]](https://resources.bio-techne.com/images/products/EEF2K-Antibody-Immunohistochemistry-Paraffin-NB100-87019-img0004.jpg "Immunohistochemistry-Paraffin: EEF2K Antibody [NB100-87019]")
Immunohistochemistry-Paraffin: EEF2K Antibody [NB100-87019]
Immunohistochemistry-Paraffin: EEF2K Antibody [NB100-87019] - Section of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eEF2 Kinase used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for EEF2K Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunoprecipitation Not recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: EEF2K
Alternate Names
Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase, EC 2.7.11, EC 2.7.11.20, eEF-2 kinase, eEF-2Kcalmodulin-dependent protein kinase III, elongation factor-2 kinase, eukaroytic elongation factor 2 kinase, eukaryotic elongation factor 2 kinase, eukaryotic elongation factor-2 kinase, HSU93850, MGC45041
Entrez Gene IDs
29904 (Human)
Gene Symbol
EEF2K
UniProt
Additional EEF2K Products
Product Documents for EEF2K Antibody - BSA Free
Product Specific Notices for EEF2K Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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