E6AP/UBE3A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-240
![Western Blot: E6AP/UBE3A Antibody [NB500-240]](https://resources.bio-techne.com/images/products/E6AP-UBE3A-Antibody-Western-Blot-NB500-240-img0002.jpg "Western Blot: E6AP/UBE3A Antibody [NB500-240]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide mapped to a region between residue 400 and 450 of human Ubiquitin Protein Ligase E3A using the numbering given in entry NP_000453.2 (GeneID 7337).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for E6AP/UBE3A Antibody - BSA Free
Western Blot: E6AP/UBE3A Antibody [NB500-240]
Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).Applications for E6AP/UBE3A Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
1-4 ug/mg of lysate
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: E6AP/UBE3A
Long Name
Human Papillomavirus E6-associated Protein
Alternate Names
ANCR, EPVE6AP, HPVE6A, UBE3A
Gene Symbol
UBE3A
UniProt
Additional E6AP/UBE3A Products
Product Documents for E6AP/UBE3A Antibody - BSA Free
Product Specific Notices for E6AP/UBE3A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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