E6AP/UBE3A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-239
![Western Blot: E6AP/UBE3A Antibody [NB500-239]](https://resources.bio-techne.com/images/products/E6AP-UBE3A-Antibody-Western-Blot-NB500-239-img0004.jpg "Western Blot: E6AP/UBE3A Antibody [NB500-239]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Canine (100%), Equine (100%), Guinea Pig (100%), Monkey (100%), Primate (100%), Rabbit (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to a portion of human ubiquitin protein ligase E3A encoded within exon 6 (LocusLink ID 7337).
Reactivity Notes
Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crabeating macaque, Naked mole rat, Thirteen-lined ground squirrel and Northern white-cheeked gibbon.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for E6AP/UBE3A Antibody - BSA Free
Western Blot: E6AP/UBE3A Antibody [NB500-239]
Western Blot: E6AP/UBE3A Antibody [NB500-239] - Detection of Human and Mouse E6AP by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-E6AP antibody NB500-239 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Western Blot: E6AP/UBE3A Antibody [NB500-239]](https://resources.bio-techne.com/images/products/E6AP-UBE3A-Antibody-Western-Blot-NB500-239-img0002.jpg "Western Blot: E6AP/UBE3A Antibody [NB500-239]")
Western Blot: E6AP/UBE3A Antibody [NB500-239]
Western Blot: E6AP/UBE3A Antibody [NB500-239] - Cytoplasmic (S100) fraction (10 mg) from HeLa cells. Antibody used at 20 ug/10 mg extract.Applications for E6AP/UBE3A Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
1 - 4 ug/mg of lysate
Western Blot
1:1000 - 1:5000
Application Notes
It recognizes a band at ~104 kDa in Western blot, representing E6AP (in cytosolic or nuclear extracts). *The investigator should determine the optimal working dilution for a specific application.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: E6AP/UBE3A
Long Name
Human Papillomavirus E6-associated Protein
Alternate Names
ANCR, EPVE6AP, HPVE6A, UBE3A
Entrez Gene IDs
7337 (Human)
Gene Symbol
UBE3A
Additional E6AP/UBE3A Products
Product Documents for E6AP/UBE3A Antibody - BSA Free
Product Specific Notices for E6AP/UBE3A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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