E2F8 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52650
![Western Blot: E2F8 Antibody [NBP1-52650] Western Blot: E2F8 Antibody [NBP1-52650]](https://resources.bio-techne.com/images/products/E2F8-Antibody-Western-Blot-NBP1-52650-img0006.jpg)
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 817 and 867 of human E2F Transcription Factor 8 using the numbering given in entry NP_078956.2 (GeneID 79733).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for E2F8 Antibody - BSA Free
Western Blot: E2F8 Antibody [NBP1-52650]
Western Blot: E2F8 Antibody [NBP1-52650] - Detection of human E2F8 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F8 antibody NBP1-52650 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: E2F8 Antibody [NBP1-52650]
Immunoprecipitation: E2F8 Antibody [NBP1-52650] - Detection of human E2F8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F8 antibody NBP1-52650(lot NBP1-52650-3) used for IP at 6 ug per reaction. E2F8 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-52650-2). For blotting immunoprecipitated E2F8, NBP1-52650 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Applications for E2F8 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: E2F8
Alternate Names
E2F family member 8, E2F transcription factor 8, E2F-8, FLJ23311, transcription factor E2F8
Gene Symbol
E2F8
Additional E2F8 Products
Product Documents for E2F8 Antibody - BSA Free
Product Specific Notices for E2F8 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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