E2F7 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57294
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: MEVNCLTLKDLISPRQPRLDFAVEDGENAQKENIFVDRSRMAPKTPIKNEPIDLSKQKKFTPERNPITPVKLVDRQQAEPW
Reactivity Notes
Mouse 87%, Rat 88%
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for E2F7 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: E2F7 Antibody [NBP2-57294]
Immunocytochemistry/Immunofluorescence: E2F7 Antibody [NBP2-57294] - Staining of human cell line U-251 MG shows localization to nuclear speckles. Antibody staining is shown in green.Chromatin Immunoprecipitation-exo-Seq: E2F7 Antibody - BSA Free [NBP2-57294]
ChIP-Exo-Seq composite graph for Anti-E2F7 (NBP2-57294) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for E2F7 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1-10µg per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: E2F7
Alternate Names
E2F transcription factor 7, E2F-7, FLJ12981, transcription factor E2F7
Gene Symbol
E2F7
Additional E2F7 Products
Product Documents for E2F7 Antibody - BSA Free
Product Specific Notices for E2F7 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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