dsRNA Antibody (K1) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11397
Conjugate
Catalog #
Key Product Details
Species Reactivity
Multi-Species
Applications
ELISA, Flow Cytometry, Immunoaffinity Purification, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # K1
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clone.
Specificity
This antibody recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNArecognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by this antibody.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
After reconstitution antibodies should be aliquoted and stored at -20C or -70C. After adding 10 mM sodium azide undiluted antibody can also be stored at 4C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided. When kept lyophilized the product will remain stable for at least 5 years at -20C or -70C. This ds-RNA antibody was manufactured by Scicon.
Applications for dsRNA Antibody (K1) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunoaffinity Purification
Optimal dilutions of this antibody should be experimentally determined.
Immunoblotting
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody can be used for ELISA, dsRNA-immunoblotting, immuno-affinity-chromatography and in certain systems also for immunohistochemistry. The optimum working dilution of the antibody for any specific application should be established by titration.
Please note that nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gel electrophoresis, because the sensitivity of detection is considerably lower after blotting from agarose gels.
Not for use for clinical purposes. For in vitro use only.
Immuno-affinity-chromatography.
Please note that nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gel electrophoresis, because the sensitivity of detection is considerably lower after blotting from agarose gels.
Not for use for clinical purposes. For in vitro use only.
Immuno-affinity-chromatography.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
The lyophilised sample should be reconstituted with 200 ul (500 ul for 500 ug size) sterile distilled water. The mAb will then be in PBS without any stabilisers or preservatives at a concentration of 1 mg/ml.
Formulation
Lyophilised from PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at -20C prior to opening. Aliquot reconstituted liquid and and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing.
Background: dsRNA
Alternate Names
Double-stranded RNA
Additional dsRNA Products
Product Documents for dsRNA Antibody (K1) - Azide and BSA Free
Product Specific Notices for dsRNA Antibody (K1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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