DOK7 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32465
![Western Blot: DOK7 Antibody [NBP2-32465]](https://resources.bio-techne.com/images/products/DOK7-Antibody-Western-Blot-NBP2-32465-img0010.jpg "Western Blot: DOK7 Antibody [NBP2-32465]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SSLDVWRATDELGSLLSLPAAGAPEPSLCTCLPGTVEYQVPTSLRAHYDTPRSLCLAPRDHS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DOK7 Antibody - BSA Free
![Western Blot: DOK7 Antibody [NBP2-32465]](https://resources.bio-techne.com/images/products/DOK7-Antibody-Western-Blot-NBP2-32465-img0009.jpg "Western Blot: DOK7 Antibody [NBP2-32465]")
Western Blot: DOK7 Antibody [NBP2-32465]
Western Blot: DOK7 Antibody [NBP2-32465] - Analysis in human cell line MCF-7 and human cell line SK-MEL-30.![Immunocytochemistry/ Immunofluorescence: DOK7 Antibody [NBP2-32465]](https://resources.bio-techne.com/images/products/DOK7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32465-img0007.jpg "Immunocytochemistry/ Immunofluorescence: DOK7 Antibody [NBP2-32465]")
Immunocytochemistry/ Immunofluorescence: DOK7 Antibody [NBP2-32465]
Immunocytochemistry/Immunofluorescence: DOK7 Antibody [NBP2-32465] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.![Immunohistochemistry-Paraffin: DOK7 Antibody [NBP2-32465]](https://resources.bio-techne.com/images/products/DOK7-Antibody-Immunohistochemistry-Paraffin-NBP2-32465-img0008.jpg "Immunohistochemistry-Paraffin: DOK7 Antibody [NBP2-32465]")
Immunohistochemistry-Paraffin: DOK7 Antibody [NBP2-32465]
Immunohistochemistry-Paraffin: DOK7 Antibody [NBP2-32465] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.Applications for DOK7 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DOK7
DOK7 (Downstream of kinase 7) is a 55 kDa member of the DOK family of cytoplasmic adaptor proteins. It links the acetylcholine receptor and the receptor tyrosine kinase MuSK in skeletal and cardiac muscle. Mutations can cause familial myasthenic syndromes. DOK7 contains pleckstrin homology, phosphotyrosine-binding and SH2 domains, and a C-terminal nuclear export signal. Multiple isoforms exist.
Long Name
Downstream of Tyrosine Kinase 7
Alternate Names
C4orf25, CMS1B
Gene Symbol
DOK7
Additional DOK7 Products
Product Documents for DOK7 Antibody - BSA Free
Product Specific Notices for DOK7 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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