DOK7 Antibody (OTI1A9) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72494
![Western Blot: DOK7 Antibody (OTI1A9)Azide and BSA Free [NBP2-72494]](https://resources.bio-techne.com/images/products/DOK7-Antibody-OTI1A9-Azide-and-BSA-Free-Western-Blot-NBP2-72494-img0001.jpg "Western Blot: DOK7 Antibody (OTI1A9)Azide and BSA Free [NBP2-72494]")
![Western Blot: DOK7 Antibody (OTI1A9)Azide and BSA Free [NBP2-72494]](https://resources.bio-techne.com/images/products/DOK7-Antibody-OTI1A9-Azide-and-BSA-Free-Western-Blot-NBP2-72494-img0002.jpg "Western Blot: DOK7 Antibody (OTI1A9)Azide and BSA Free [NBP2-72494]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1A9
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human DOK7(NP_775931) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
52.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DOK7 Antibody (OTI1A9) - Azide and BSA Free
Western Blot: DOK7 Antibody (OTI1A9)Azide and BSA Free [NBP2-72494]
Western Blot: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK7.![Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]](https://resources.bio-techne.com/images/products/DOK7-Antibody-OTI1A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72494-img0008.jpg "Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]")
Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]
Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494] - Staining of paraffin-embedded Human pancreas tissue using anti-DOK7 mouse monoclonal antibody.![Flow Cytometry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]](https://resources.bio-techne.com/images/products/DOK7-Antibody-OTI1A9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72494-img0004.jpg "Flow Cytometry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]")
Flow Cytometry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]
Flow Cytometry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK7.![Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]](https://resources.bio-techne.com/images/products/DOK7-Antibody-OTI1A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72494-img0005.jpg "Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]")
![Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]](https://resources.bio-techne.com/images/products/DOK7-Antibody-OTI1A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72494-img0006.jpg "Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]")
![Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]](https://resources.bio-techne.com/images/products/DOK7-Antibody-OTI1A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72494-img0007.jpg "Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]")
![Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]](https://resources.bio-techne.com/images/products/DOK7-Antibody-OTI1A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72494-img0009.jpg "Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]")
![Flow Cytometry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]](https://resources.bio-techne.com/images/products/DOK7-Antibody-OTI1A9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72494-img0003.jpg "Flow Cytometry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494]")
Applications for DOK7 Antibody (OTI1A9) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DOK7
DOK7 (Downstream of kinase 7) is a 55 kDa member of the DOK family of cytoplasmic adaptor proteins. It links the acetylcholine receptor and the receptor tyrosine kinase MuSK in skeletal and cardiac muscle. Mutations can cause familial myasthenic syndromes. DOK7 contains pleckstrin homology, phosphotyrosine-binding and SH2 domains, and a C-terminal nuclear export signal. Multiple isoforms exist.
Long Name
Downstream of Tyrosine Kinase 7
Alternate Names
C4orf25, CMS1B
Gene Symbol
DOK7
Additional DOK7 Products
Product Documents for DOK7 Antibody (OTI1A9) - Azide and BSA Free
Product Specific Notices for DOK7 Antibody (OTI1A9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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