DOCK2 Antibody (JE53-62)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32276
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE53-62 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human DOCK2 aa 1,781-1,830 / 1,830. (Uniprot: Q92608)
Localization
Endomembrane system, Cytoplasm, cytoskeleton.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
212 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DOCK2 Antibody (JE53-62)
![DOCK2 Antibody (JE53-62) Western Blot: DOCK2 Antibody (JE53-62) [NBP3-32276]](https://resources.bio-techne.com/images/products/nbp3-32276_-western-blot-639204597147390679.jpg "Western Blot: DOCK2 Antibody (JE53-62) [NBP3-32276]")
Western Blot: DOCK2 Antibody (JE53-62) [NBP3-32276]
Western blot analysis on different lysates with NBP3-32276 at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: THP-1 cell lysate Lane 3: Raji cell lysate Lane 4: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 212 kDa Observed band size: 180 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32276 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.Applications for DOCK2 Antibody (JE53-62)
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DOCK2
Long Name
Dedicator of Cytokinesis Protein 2
Alternate Names
dedicator of cytokinesis 2, dedicator of cytokinesis protein 2, FLJ46592, KIAA0209dedicator of cyto-kinesis 2
Gene Symbol
DOCK2
Additional DOCK2 Products
Product Documents for DOCK2 Antibody (JE53-62)
Product Specific Notices for DOCK2 Antibody (JE53-62)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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