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DNMT3B Antibody

Catalog # NB300-516 | Novus Biologicals a Bio-Techne Brand
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NB300-516
NB300-516-0.025mg

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

Product Summary for DNMT3B Antibody

Immunogen

Synthetic peptide corresponding to the residues M(1) K G D S R H L N E E E G A(14) C of mouse DNMT3b

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34874218) Human reactivity reported in scientific literature (PMID: 20847044). Mouse reactivity reported in scientific literature (PMID: 21149390)..

Localization

Nuclear

Specificity

This detects DNA methyltransferase 3b (Dnmt3b) from human and mouse tissues and cells as well as recombinant human Dnmt3b. This detects, to a lesser extent, full-length human recombinant Dnmt3a.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNMT3B Antibody

Western Blot: DNMT3B Antibody [NB300-516] - Expression level of DNMT3B by RT-PCR and Western blot (W.B.) analysis in human oral keratinocyte (HOK) and OSCC cell lines. GAPDH and a-tubulin were used as internal control, respectively. Image collected and cropped by CiteAb from the following publication (jbiomedsci.biomedcentral.com/articles/10.1186/s12929-020-00644-z), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left).
Western Blot: DNMT3B Antibody [NB300-516] - Homogenized mouse brain cells. WB image submitted by a verified customer review.

Applications for DNMT3B Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:1000

Simple Western

20 ug/mL

Western Blot

2.0 ug/mL
Application Notes
May be Useful in WB. ChIP usage was reported in scientific literature. WB: Detects a 130 kDa band is seen in P19 nuclear extracts.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 3.7 using NB300-516 in the following applications:

Published Applications

Read 16 publications using NB300-516 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNMT3B

DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation. Hypermethylation of DNA has also been implicated in tumorigenesis through improper methylation of growth regulatory gene promoter regions.

Long Name

DNA [Cytosine-5-]-Methyltransferase 3B

Alternate Names

ICF1, M.HsaIIIB

Entrez Gene IDs

1789 (Human); 13436 (Mouse)

Gene Symbol

DNMT3B

UniProt

Product Documents for DNMT3B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNMT3B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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