DNA Polymerase beta Antibody (HL1512)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48656
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1512
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human DNA Polymerase beta. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit DNA Polymerase beta Antibody (HL1512) (NBP3-48656) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for DNA Polymerase beta Antibody (HL1512)
Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody (HL1512) [NBP3-48656] -
DNA Polymerase beta antibody [HL1512] detects DNA Polymerase beta protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse esophagus.
DNA Polymerase beta stained by DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: DNA Polymerase beta Antibody (HL1512) [NBP3-48656] -
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: DNA Polymerase beta Antibody (HL1512) [NBP3-48656] -
DNA Polymerase beta antibody [HL1512] detects DNA Polymerase beta protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 10 umin.Green: DNA Polymerase beta stained by DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:250.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10 um.
Applications for DNA Polymerase beta Antibody (HL1512)
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DNA Polymerase beta
Alternate Names
POLB
Gene Symbol
POLB
Additional DNA Polymerase beta Products
Product Documents for DNA Polymerase beta Antibody (HL1512)
Product Specific Notices for DNA Polymerase beta Antibody (HL1512)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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