PKM2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) conjugated to Digoxigenin at 10 µg/mL overnight at 4 °C, followed by Mouse Anti-Digoxigenin Recombinant Monoclonal Antibody (Catalog # MAB7520R) at 5 µg/mL for 1 hour at room temperature. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Digoxigenin Antibody

Application

Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) conjugated to Digoxigenin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Digoxigenin

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

References

Décarie, A.A. et al. (1994) Peptides 15(3):511.
Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.

Alternate Names

DIG

Additional Digoxigenin Products

Product Documents for Digoxigenin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Digoxigenin Antibody

For research use only

Citations
Reviews

Digoxigenin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7520R

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Digoxigenin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Digoxigenin Antibody

Digoxigenin-Conjugated Antibody in Human Prostate Cancer Tissue.