Digoxigenin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7520R
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Multi-Species
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2A Clone # 611621R
Product Specifications
Immunogen
KLH-coupled Digoxigenin
Specificity
Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Digoxigenin Antibody
Digoxigenin-Conjugated Antibody in Human Prostate Cancer Tissue.
PKM2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) conjugated to Digoxigenin at 10 µg/mL overnight at 4 °C, followed by Mouse Anti-Digoxigenin Recombinant Monoclonal Antibody (Catalog # MAB7520R) at 5 µg/mL for 1 hour at room temperature. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Digoxigenin Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) conjugated to Digoxigenin
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) conjugated to Digoxigenin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5
mg/mL in sterile PBS.
For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Digoxigenin
References
- Décarie, A.A. et al. (1994) Peptides 15(3):511.
- Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
- Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.
Alternate Names
DIG
Additional Digoxigenin Products
Product Documents for Digoxigenin Antibody
Product Specific Notices for Digoxigenin Antibody
For research use only
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