DDIT4 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-22966
![Western Blot: DDIT4 Antibody [NBP1-22966]](https://resources.bio-techne.com/images/products/DDIT4-Antibody-Western-Blot-NBP1-22966-img0006.jpg "Western Blot: DDIT4 Antibody [NBP1-22966]")
![Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody [NBP1-22966]](https://resources.bio-techne.com/images/products/DDIT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-22966-img0003.jpg "Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody [NBP1-22966]")
![Western Blot: DDIT4 Antibody [NBP1-22966]](https://resources.bio-techne.com/images/products/DDIT4-Antibody-Western-Blot-NBP1-22966-img0005.jpg "Western Blot: DDIT4 Antibody [NBP1-22966]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%), Chimpanzee (100%), Equine (100%), Monkey (100%), Orangutan (100%), Primate (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human protein regulated in development and DNA damage response 1 (DNA-damage-inducible transcript 4) using the numbering given in entry NP_061931.1 (GeneID 54541).
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 25867045)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DDIT4 Antibody - BSA Free
Western Blot: DDIT4 Antibody [NBP1-22966]
DDIT4-Antibody-Western-Blot-NBP1-22966-img0005.jpg![Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody [NBP1-22966]](https://resources.bio-techne.com/images/products/DDIT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-22966-img0004.jpg "Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody [NBP1-22966]")
Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody [NBP1-22966]
Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-22966] - HeLa cells stained with NBP1-22966 after 0.2% hypoxia or CoCl2 overnight. Image from verified customer review.![Western Blot: DDIT4 Antibody [NBP1-22966]](https://resources.bio-techne.com/images/products/DDIT4-Antibody-Western-Blot-NBP1-22966-img0002.jpg "Western Blot: DDIT4 Antibody [NBP1-22966]")
Western Blot: DDIT4 Antibody [NBP1-22966]
Western Blot: DDIT4 Antibody [NBP1-22966] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Lysate was prepared from cells that had been treated with cobalt chloride (A and B) or mock treated (A). NBP1-22966 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate.Applications for DDIT4 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500 to 1:2000
Immunoprecipitation
5-15 ug/mg lysate
Western Blot
1:1000-1:4000
Application Notes
Formaldehyde fixation is recommended. Permeabilization with Triton X-100 is recommended for formaldehydefixed cells.
Reviewed Applications
Read 1 review rated 4 using NBP1-22966 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DDIT4
Alternate Names
Dig2, DNA-damage-inducible transcript 4, FLJ20500, HIF-1 responsive RTP801 (RTP801), Protein regulated in development and DNA damage response 1, REDD-1DNA damage-inducible transcript 4 protein, REDD1HIF-1 responsive protein RTP801, RTP801RP11-442H21.1
Entrez Gene IDs
54541 (Human)
Gene Symbol
DDIT4
UniProt
Additional DDIT4 Products
Product Documents for DDIT4 Antibody - BSA Free
Product Specific Notices for DDIT4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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