DACH1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85320
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (91%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PKRTQSVTSPENSHIMPHSVPGLMSPGIIPPTGLTAAAAAAAAATNAAIAEAMKVKKIKLEAMSNYHASNNQHGADSENGDMNSSVGSSDGSWDKETLPSSPSQGPQASITHPRMPGARSLPLSHPLNHLQQSHLLPNGLELP
Reactivity Notes
Rat (88%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit DACH1 Antibody - BSA Free (NBP1-85320) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-DACH1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for DACH1 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: DACH1 Antibody [NBP1-85320]](https://resources.bio-techne.com/images/products/DACH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85320-img0006.jpg "Immunocytochemistry/ Immunofluorescence: DACH1 Antibody [NBP1-85320]")
Immunocytochemistry/ Immunofluorescence: DACH1 Antibody [NBP1-85320]
Immunocytochemistry/Immunofluorescence: DACH1 Antibody [NBP1-85320] - Staining of human cell line A-431 shows localization to nucleoplasm & vesicles.![Immunohistochemistry-Paraffin: DACH1 Antibody [NBP1-85320]](https://resources.bio-techne.com/images/products/DACH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85320-img0007.jpg "Immunohistochemistry-Paraffin: DACH1 Antibody [NBP1-85320]")
Immunohistochemistry-Paraffin: DACH1 Antibody [NBP1-85320]
Immunohistochemistry-Paraffin: DACH1 Antibody [NBP1-85320] - Staining of human small intestine shows strong nuclear positivity in glandular cells.![DACH1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: DACH1 Antibody - BSA Free [NBP1-85320]](https://resources.bio-techne.com/images/products/nbp1-85320_rabbit-polyclonal-dach1-antibody-2562025911613.jpg "Chromatin Immunoprecipitation-exo-Seq: DACH1 Antibody - BSA Free [NBP1-85320]")
Chromatin Immunoprecipitation-exo-Seq: DACH1 Antibody - BSA Free [NBP1-85320]
ChIP-Exo-Seq composite graph for Anti-DACH1 (NBP1-85320) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for DACH1 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DACH1
Alternate Names
Dach1, DACHdachshund homolog 1, dachshund homolog (Drosophila), dachshund homolog 1 (Drosophila), FLJ10138
Gene Symbol
DACH1
Additional DACH1 Products
Product Documents for DACH1 Antibody - BSA Free
Product Specific Notices for DACH1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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