Cytokeratin 8 Antibody (SU0338)

Western Blot: Cytokeratin 8 Antibody (SU0338) [NBP2-67468]

Western Blot: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of Cytokeratin 8 on different lysates with Rabbit anti-Cytokeratin 8 antibody at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468]

Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of SK-Br-3 cells labeling Cytokeratin 8 (and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody Cytokeratin 8 (red) at 1/50 dilution and Beta-tubulin (yellow) at 1/500 dilution at +4 overnight. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 8 (washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (red) at 1/50 dilution and Vimentin (green) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).