Cytokeratin 8/18 Antibody (KRT8.18/6579R) [mFluor Violet 500 SE]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14099MFV500
Recombinant Monoclonal Antibody
![Cytokeratin 8/18 Antibody (KRT8.18/6579R) [mFluor Violet 500 SE]](https://resources.bio-techne.com/images/products/nbp3-14099mfv500_rabbit-cytokeratin-8-18-mab-krt8-18-6579r-mfv500-se-5720231350290.png "Cytokeratin 8/18 Antibody (KRT8.18/6579R) [mFluor Violet 500 SE] -")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # KRT8.18/6579R
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full-length human Cytokeratin 8 and Cytokeratin 18 proteins
Localization
Cytoplasm
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This monoclonal antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained. Immunohistochemical staining with this monoclonal antibody is indistinguishable from that obtained with monoclonal antibody 5D3.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for Cytokeratin 8/18 Antibody (KRT8.18/6579R) [mFluor Violet 500 SE]
Cytokeratin 8/18 Antibody (KRT8.18/6579R) [mFluor Violet 500 SE] -
Cytokeratin 8/18 Antibody (KRT8.18/6579R) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.Applications for Cytokeratin 8/18 Antibody (KRT8.18/6579R) [mFluor Violet 500 SE]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Cytokeratin 8/18
Alternate Names
CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, Keratin-8, KO, Type-II keratin Kb8
Gene Symbol
KRT8
Additional Cytokeratin 8/18 Products
Product Documents for Cytokeratin 8/18 Antibody (KRT8.18/6579R) [mFluor Violet 500 SE]
Product Specific Notices for Cytokeratin 8/18 Antibody (KRT8.18/6579R) [mFluor Violet 500 SE]
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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