Cytokeratin 5 Antibody (GT1486) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13606

Novus Biologicals, part of Bio-Techne
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NBP3-13606
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (97%), Canine (97%), Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT1486

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 5 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 5. The exact sequence is proprietary.

Reactivity Notes

Chicken (80%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Cytokeratin 5 Antibody (GT1486) - Azide and BSA Free

Western Blot: Cytokeratin 5 Antibody (GT1486) [NBP3-13606]

Western Blot: Cytokeratin 5 Antibody (GT1486) [NBP3-13606]

Western Blot: Cytokeratin 5 Antibody (GT1486) [NBP3-13606] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 5 antibody [GT1486] (NBP3-13606) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (GT1486) [NBP3-13606]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (GT1486) [NBP3-13606]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (GT1486) [NBP3-13606] - Cytokeratin 5 antibody [GT1486] detects Cytokeratin 5 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat thymus gland. Cytokeratin 5 stained by Cytokeratin 5 antibody [GT1486] (NBP3-13606) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (GT1486) [NBP3-13606]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (GT1486) [NBP3-13606]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (GT1486) [NBP3-13606] - Cytokeratin 5 antibody [GT1486] detects Cytokeratin 5 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. Cytokeratin 5 stained by Cytokeratin 5 antibody [GT1486] (NBP3-13606) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Cytokeratin 5 Antibody (GT1486) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000-1:10000

Immunohistochemistry-Paraffin

1:1000-1:10000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 5

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Alternate Names

CK-5, DDD, EBS2, Keratin 5, KRT5

Gene Symbol

KRT5

Additional Cytokeratin 5 Products

Product Documents for Cytokeratin 5 Antibody (GT1486) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 5 Antibody (GT1486) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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