Cytokeratin 5 Antibody (CL9297) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44236

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-44236
Print Quote
Bulk Order
Forumulation
Catalog #
BSA Free
NBP2-88923
View Product
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL9297

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Cytokeratin 5 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P13647, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Cytokeratin 5 Antibody (CL9297) - Azide and BSA Free (NBP2-88923) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Cytokeratin 5 Antibody (CL9297) - Azide and BSA Free

Cytokeratin 5 Antibody (CL9297) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody [NBP3-44236]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody [NBP3-44236]

Staining of A-431 cells using the Anti-Cytokeratin 5 monoclonal antibody) .
Cytokeratin 5 Antibody (CL9297) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP3-44236]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP3-44236]

Analysis in human prostate and kidney tissues using NBP3-44236 antibody. Corresponding Cytokeratin 5 RNA-seq data are presented for the same tissues.
Cytokeratin 5 Antibody (CL9297) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP3-44236]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP3-44236]

Staining of human cervix shows moderate to strong cytoplasmic positivity in squamous epithelial cells.
View 5 more images

Applications for Cytokeratin 5 Antibody (CL9297) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 5

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Alternate Names

CK-5, DDD, EBS2, Keratin 5, KRT5

Gene Symbol

KRT5

Additional Cytokeratin 5 Products

Product Documents for Cytokeratin 5 Antibody (CL9297) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 5 Antibody (CL9297) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.