Cytokeratin 5/6/18 Antibody (SPM267) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08546
![Immunohistochemistry-Paraffin: Cytokeratin 5/6/18 Antibody (SPM267) - Azide and BSA Free [NBP3-08546]](https://resources.bio-techne.com/images/products/Cytokeratin-5-6-18-Antibody-SPM267-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08546-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/6/18 Antibody (SPM267) - Azide and BSA Free [NBP3-08546]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Bovine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM267
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Detergent-insoluble fraction of psoriatic human epidermis
Localization
Cytoplasmic
Specificity
It recognizes polypeptides of 58kDa, 56kDa, and 45kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This monoclonal antibody shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This monoclonal antibody is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07606).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 5/6/18 Antibody (SPM267) - Azide and BSA Free
Immunohistochemistry-Paraffin: Cytokeratin 5/6/18 Antibody (SPM267) - Azide and BSA Free [NBP3-08546]
Immunohistochemistry-Paraffin: Cytokeratin 5/6/18 Antibody (SPM267) - Azide and BSA Free [NBP3-08546] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 5/6/18 Mouse Monoclonal Antibody (SPM267).Applications for Cytokeratin 5/6/18 Antibody (SPM267) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Cytokeratin 5/6/18
Alternate Names
58 kDa cytokeratin, CK5, CK-5, cytokeratin-5, DDD, EBS2, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, K5, keratin 5, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes), keratin, type II cytoskeletal 5, Keratin-5, KRT5A, Type-II keratin Kb5
Gene Symbol
KRT5
Additional Cytokeratin 5/6/18 Products
Product Documents for Cytokeratin 5/6/18 Antibody (SPM267) - Azide and BSA Free
Product Specific Notices for Cytokeratin 5/6/18 Antibody (SPM267) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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