Cytokeratin 5/6/18 Antibody (SPM267)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07606
![Immunohistochemistry-Paraffin: Cytokeratin 5/6/18 Antibody (SPM267) [NBP3-07606]](https://resources.bio-techne.com/images/products/Cytokeratin-5-6-18-Antibody-SPM267-Immunohistochemistry-Paraffin-NBP3-07606-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/6/18 Antibody (SPM267) [NBP3-07606]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Bovine
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM267
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Detergent-insoluble fraction of psoriatic human epidermis
Localization
Cytoplasmic
Specificity
It recognizes polypeptides of 58kDa, 56kDa, and 45kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This monoclonal antibody shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This monoclonal antibody is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08546)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 5/6/18 Antibody (SPM267)
Immunohistochemistry-Paraffin: Cytokeratin 5/6/18 Antibody (SPM267) [NBP3-07606]
Immunohistochemistry-Paraffin: Cytokeratin 5/6/18 Antibody (SPM267) [NBP3-07606] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 5/6/18 Mouse Monoclonal Antibody (SPM267).Applications for Cytokeratin 5/6/18 Antibody (SPM267)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 5/6/18
Alternate Names
58 kDa cytokeratin, CK5, CK-5, cytokeratin-5, DDD, EBS2, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, K5, keratin 5, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes), keratin, type II cytoskeletal 5, Keratin-5, KRT5A, Type-II keratin Kb5
Gene Symbol
KRT5
Additional Cytokeratin 5/6/18 Products
Product Documents for Cytokeratin 5/6/18 Antibody (SPM267)
Product Specific Notices for Cytokeratin 5/6/18 Antibody (SPM267)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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