Cytokeratin 16 Antibody (8L6R4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16678
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8L6R4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 16 (P08779). MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSRFSSGGACGLGGGYGGGFSSSSSFGSGFGGGYGGGLGAGFGGGLGA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytokeratin 16 Antibody (8L6R4)
![Western Blot: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678]](https://resources.bio-techne.com/images/products/Cytokeratin-16-Antibody-8L6R4-Western-Blot-NBP3-16678-img0005.jpg "Western Blot: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678]")
Western Blot: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678]
Western Blot: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678] - Western blot analysis of extracts of Mouse large intestine cells, using Cytokeratin 16 antibody (NBP3-16678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678] -
Immunohistochemistry: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Cytokeratin 16 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678] -
Immunohistochemistry: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Cytokeratin 16 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.Applications for Cytokeratin 16 Antibody (8L6R4)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:100 - 1:400
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cytokeratin 16
Alternate Names
CK16, CK-16, cytokeratin 16, Cytokeratin-16, FNEPPK, focal non-epidermolytic palmoplantar keratoderma, K16, K1CP, keratin 16, keratin, type I cytoskeletal 16, keratin-16, KRT16A, NEPPK
Gene Symbol
KRT16
Additional Cytokeratin 16 Products
Product Documents for Cytokeratin 16 Antibody (8L6R4)
Product Specific Notices for Cytokeratin 16 Antibody (8L6R4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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